We selected the top 5 mutated genes in liver (TERT, CTNNB1, TP53, ALB, ARID1A, [41]) and top 4 mutated genes in colon (APC, TP53, SYNE1, PIK3CA, [2]) cancer according to two recent studies and analyzed the mean coverage in FF, FFPE and for the three FFPE extraction kits for all colon and liver tumor samples (S5 Table). This evidence concerns the gene PIK3CA and cancer.