TP53 and cancer: We selected the top 5 mutated genes in liver (TERT, CTNNB1, TP53, ALB, ARID1A, [41]) and top 4 mutated genes in colon (APC, TP53, SYNE1, PIK3CA, [2]) cancer according to two recent studies and analyzed the mean coverage in FF, FFPE and for the three FFPE extraction kits for all colon and liver tumor samples (S5 Table).