SQSTM1 and hydrops fetalis: CQ or Bafilomycin A1 strongly inhibited basal autophagy, marked by the accumulation of LC3 and p62, and HF treatment caused a decrease in p62 and LC3-II levels in the presence of CQ (possibly due to activation of autophago-lysosomal degradation), but not in Bafilomycin A1–treated cells (Fig 4C and S8A and S8B Fig), suggesting that Bafilomycin A1 exhibits more lysosomal inhibitory effect than CQ, as previously described [31].