In this study of genetic associations with T2D and related glycemic traits in Chinese individuals from 9 provinces in the CHNS, we observed associations with fasting glucose at SIX3-SIX2 and G6PC2, including a coding variant representing an additional signal at G6PC2. We also showed that the SIX3-SIX2 fasting glucose locus colocalizes with eQTL associations for SIX3, SIX2, SIX3-AS1, RP11-89K21. This evidence concerns the gene SIX2 and type 2 diabetes mellitus.