As shown in Figure 3, when PBMCs from normal subjects were cocultured with expanded CD4+CD25high regulatory T cells from CKD patients, a significant decrease in the percentage of IFN-γ+ Th1 (Figure 3(a)) and IL-17A+ Th17 cells (Figure 3(b)) in normal PBMCs was observed compared with no coculture with Tregs. Here, CD4 is linked to chronic kidney disease.