We also examined DNA methylation for CHKA, ETNK1, and ETNK2 genes within the IDHmut glioma population and, in line with the results in our cell models, found no correlation with mRNA z-scores (Pearson correlation coefficients of − 0.2 for CHKA, − 0.3 for ETNK1, and − 0.3 for ETNK2; Additional file 1: Figure S6d-f), suggesting that reduced CHKA and ETNK2 mRNA expression in the IDHmut glioma patient samples was not the result of 2-HG-induced DNA hypermethylation at the promoters of these genes. This evidence concerns the gene ETNK2 and glioma.