As shown in Additional file 1: Figure S6A-B, the mean z-scores for CHKA and ETNK2 mRNA expression showed a significant reduction in IDHmut glioma samples relative to IDHwt (0.21 in IDHwt to − 0.16 in IDHmut, p < 0.0001 for CHKA and 0.69 in IDHwt to − 0.12 in IDHmut, p < 0.001 for ETNK2). This evidence concerns the gene CHKA and glioma.