In this study, we aimed to determine whether in vitro concentrations of DHA (100-, 150- and 200 μM/L) equal to the levels of DHA achievable in the human body following supplementation of the diet with different amounts of omega-3 PUFAs/day are able to modulate Wt-p53, survivin, and miRNA-16-1 expression in Wt-p53/KRAS-mutant HCT-116 cells with stem cell-like properties that shows early stages of tumor initiation and development [40–43]. This evidence concerns the gene BIRC5 and neoplasm.