It should be noted that while the level of protection is small, 4T1 is considered to be a poorly immunogenic tumor as vaccination with irradiated 4T1 tumor cells fails to protect any animals from a tumor challenge [36]. Additionally, when this 4T1 autophagosome vaccine is combined with a T cell agonist, anti-OX40, therapeutic efficacy is significantly increased [24]. The gene discussed is TNFRSF4; the disease is neoplasm.