In order to determine whether the inhibitory effect of curcumol on growth of CCA cells is related to induction of apoptosis, we calculated the percentage of RBE and HCCC-9810 cells undergoing apoptosis following curcumol treatment by Annexin V-FITC /PI fluorescence staining assay (Figure 2B), curcumol (75 and 100 μg/mL, for 48 h) induced significant apoptosis in RBE and HCCC-9810 cells. The gene discussed is ANXA5; the disease is cholangiocarcinoma.