A recent study also indicates that the combination of obesity (induced by diet) with chronic unpredictable mild stress (induced by unpredictable mild stressors like 8 h of food or water deprivation, confusing day and night, soaking the cage with water or horizontal oscillation for 20 min) induces depression and anxiety-like behaviors and the down-regulation of leptin/LepRb signaling (Yang et al., 2016). This evidence concerns the gene LEP and Anxiety.