GATA4 and infection: Immunostaining (Fig. 1F) and FACS analysis (Figs S3, S4, Table 1) for ZIKV NS1 protein and the Sertoli cell marker GATA418 revealed that while >90% of the Sertoli cells were positive for GATA4, ~25–45% of the cells were positive for viral antigen 48 h post-infection.