In agreement with data shown in Fig. 2, immunofluoresence microscopy revealed that the percentage of SphK2 stained surface in neuron (nuclear and cytoplasmic compartments) was decreased (F(1,837) = 49.0; p < 0.0001; Fig. 5b) in the AD group as compared to control for all structures (frontal cortex: p = 0.033; entorhinal cortex and CA1: p < 0.0001). This evidence concerns the gene SPHK2 and Alzheimer disease.