To determine which HMGB1 receptor(s) is involved in mediating cell invasion, we silenced four well-recognized HMGB1 receptors (RAGE, TLR2, TLR4, and CD24) individually by transiently transfecting specific shRNA oligos in Panc-1 cancer cells for 48 h (Fig. 3c). Here, TLR4 is linked to cancer.