Furthermore, the number of ICOS+, Granzyme B+ TILs and the PD-1/CD8 ratio in the primary tumor were significantly correlated with the values in the metastatic tumor (r = 0.649, p = 0.001; r = 0.426, p = 0.038; r = 0.498, p = 0.013, respectively), and the number of PD-1+ TILs tended to be correlated with that in the metastatic tumor (r = 0.387, p = 0.062) (Fig. 6). Here, ICOS is linked to metastatic neoplasm.