The RT-PCR analysis showed that compared with pericarcinomatous tissues, SFRP1 expression was markedly decreased in CRC tissues, which was further confirmed by Western blot analysis (Fig. 1) SFRP1 expression was then assessed by RT-PCR in six CRC cell lines (sw-480, sw1116, caco-2, ht-29, colo-205, and hct-116); GAPDH was used as a control for both cDNA quality and efficiency of the PCR amplification. The gene discussed is GAPDH; the disease is colorectal carcinoma.