In order to illuminate the function of SGK1 in the regulation of autophagy in PCa, first, autophagy activity was evaluated with AO staining after SGK1 elimination, as shown in Fig. 3a, shSGK1 exhibited a stark increase in discrete acidic vesicles compared to LV2-Ctrl in LNCaP cells (Fig. 3b), which was further confirmed by increased LC3II protein levels both in shSGK1-LNCaP and -PC3 cells (Fig. 3f). The gene discussed is SGK1; the disease is posterior cortical atrophy.