VRK1 and infection: The most significant marker (IWB45005) was located at 158.9 cM and had sizable allelic effects ranging from -0.19 to -0.32 and relatively high R2 values from 0.07 to 0.12, the highest observed outside of Pch1. This marker was found to have a significant (p < 0.001) effect on reducing eyespot infection in the absence and presence of Pch1 (S2 Fig).