To rule out a requirement for AS Kir4.1 to maintain MN survival in an animal model of ALS, we assessed MN populations in WT, SOD1G93A (mSOD1), and AS-Kir4.1cKO, SOD1G93A (mSOD1, cKO) compound transgenic animals (Figure 4F). This evidence concerns the gene KCNJ10 and amyotrophic lateral sclerosis.