However, when MD2 or MyD88 was knocked down in the TLR4 overexpressing liver cancer stem cells, excessive TLR4 could significantly not alter the growth and the colony formation ability of liver cancer stem cell (TLR4 + MD2i:37.79 ± 3.29 vs 33.76 ± 5.333%, P = .243 > .05; TLR4 + MyD88i: 37.79 ± 3.29 vs 35.2 ± 8.67%, P = .362 > .05) (Figure S3B). This evidence concerns the gene LY96 and liver cancer.