The mitochondrial function regulators PGC‐1α, Mfn‐2, NRF1 and TFAM play critical roles in regulating mitochondria biogenesis, respiration and OXPHOS.25, 26, 27 Our in vivo data showed that the protein levels of PGC‐1α, Mfn‐2, NRF‐1 and TFAM were significantly decreased in patients and cows with NASH (Figure 2A‐D). Here, NRF1 is linked to metabolic dysfunction-associated steatohepatitis.