In the present study, we generated heat shock-conditioned tumor lysate for GBC (M2), which have some important characteristics that suggest its potential as an antigen source for DC vaccines: (1) it contains a broad panel of TAAs, also expressed in tumors from GBC patients, (2) it includes different molecules that could act as DAMPs (released HMGB1, ATP and eCRT), (3) it promotes a rapid and efficient differentiation of monocytes to mature DCs, and (4) DCs generated with this lysate are able to induce the activation of T cells that specifically recognize tumor cells. Here, HMGB1 is linked to neoplasm.