CD8A and melanoma: Then, CD8+ T cells were isolated after co-culture by cell-sorting and challenged with two HLA-A2+ GBCCL present in the M2 lysate (2TKB and GBd1), a HLA-A2+ GBCCL that was not included in the M2 lysate (CAVE), a HLA-A2+ melanoma cell line (Mel1), or with K562 cells as a negative control.