In our model, it seems that serial transplantation increases the potential of reconstitution of the LSC, as we transplanted a lower number of CD34+ in the 2nd generation of mice, but obtained an increased number of blasts in mouse 3, but this was not reflected by a clonal skewing or expansion in the sequential HSC sequencing in our MDS model. This evidence concerns the gene CD34 and myelodysplastic syndrome.