Huang et al. analyzed the methylation status of GSTP1, RASSF1A, APC and Secreted Frizzled Related Protein 1 (SFRP1) genes in plasma samples of 72 patients with HCC and 37 subjects with benign liver diseases showing that RASSF1A methylation was positively correlated with tumour size, while GSTP1 methylation was associated with elevated AFP levels in the serum, and SFRP1 methylation was more common in females [27]. This evidence concerns the gene APC and hepatocellular carcinoma.