Haemotoxylin and eosin staining of the pancreas of Pdx1-Cre;LSL-KrasG12D/+;Trp53flox/flox;Ksr1-/- mice sacrificed at several time points confirmed the presence of pancreatic ductal adenocarcinoma with tumors identified in all mice between 3–4 weeks of age. Here, PDX1 is linked to pancreatic ductal adenocarcinoma.