To test this, we quantified levels of seven cytokines and chemokines (interferon-γ (IFN-γ), TNF-α, interleukin-1b (IL-1b), interleukin-6 (IL-6), MCP-1, G-CSF and GM-CSF) in the media immediately following cryptococcal infection and again 18 hours later. This evidence concerns the gene TNF and cryptococcosis.