To analyze the potential prognostic characteristics of tumor differentiation related genes in pancreatic adenocarcinoma, 6 differentially expressed genes in all different tumor differentiation grades (JUB, ERLIN1, HMGA2, FAM110B, EGFR and MCM2) and four differentially expressed genes that enriched in pancreatic adenocarcinoma signaling pathway (BCL2L1, E2F1, RAC1 and STAT1) were analyzed using the R package (3.4.0 version, 2017). The gene discussed is E2F1; the disease is pancreatic adenocarcinoma.