Having established that the expression of KDM6B was clearly the highest in t(8;21) AML patients amongst all the AML subtypes (Fig. 1a) and Kasumi-1 is an AML cell line with AML1-ETO fusion gene (Asou et al. 1991), we then focused the following studies with Kasumi-1 to investigate the effects of GSK-J4 on leukemic cells in vitro and in vivo. Here, RUNX1 is linked to acute myeloid leukemia.