Luciferase assay results indicated that LiCl could increase the TCF/LEF promoter activities by near 1300-fold compared with its mock, and P1 infection significantly abolished the activation of TCF/LEF promoter by LiCl (P < 0.05) and the activity was reduced to half of pGL-TCF/LEF+LiCl group (Figure 5), further suggested that Wnt signaling pathway was inhibited by P1 infection in vitro. Here, HNF4A is linked to infection.