To further examine whether other key autophagy pathway genes participate in suppression of viral infection, we obtained Arabidopsis knockdown mutants of genes PI3K and VPS15 and Arabidopsis knockout mutants of genes ATG2, ATG5, and ATG7 and also knocked down these genes in N. benthamiana plants using the TRV vector. The gene discussed is PIK3R4; the disease is viral infectious disease.