ITGAE and neoplasm: To verify that the reduced number of cDC1s in the poly I:C plug was due to a decreased pool of circulating pre-DCs rather than changes in recruitment cytokines, we measured recruitment of CD103+ cDC1s to a matrigel plug containing the recruitment factor C-C motif chemokine ligand 4 (Ccl4), which is known to be downregulated in some tumor types26.