Compared with control HFs, we observed a significant reduction in the percentage of MKs that were positive for the proliferation marker, Ki-67 (red, Alexa555), in siATG5-treated HFs, with a significantly higher percentage of apoptotic (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling [TUNEL]-positive) cells (green, Alexa488) (Fig 5E–5G). The gene discussed is MKI67; the disease is Hand-foot syndrome.