Stimulation of AMPK was still observed after 6 h incubation with canagliflozin or A769662, as assessed by phosphorylation of the AMPK substrate ACC and infection with Ad.AMPK-DN markedly suppressed canagliflozin- and A769662-stimulated ACC phosphorylation (Supplementary Figure S3). The gene discussed is PRKAA2; the disease is infection.