ANXA5 and infection: To determine whether infection with wild-type S. Javiana resulted in cell death (through apoptotic and other cell death pathways), we stained HIEC-6 cells infected with wild-type and ΔcdtB S. Javiana strains with annexin V to detect apoptotic cells and with propidium iodide (PI) to detect dead cells, regardless of the pathway leading to cell death (Fig. 6A).