CK5/6 IHC was performed by using FLEX Monoclonal Mouse Anti-human Cytokeratin 5/6, clone D5/16 B4 (Lot No. 20042129) by DAKO envision method according to manufacturers protocol on 127 cases of urothelial carcinoma (on representative tissue blocks). This evidence concerns the gene KRT5 and urothelial carcinoma.