In order to identify whether the decrease of RT product in HCT116 p53+/+ cells was either due to the inhibition in the process in viral cDNA synthesis by reverse transcriptase or because of possible degradation of virus cDNA by nucleases in infected cells, the amount of remaining late RT product was quantified at 1.5 h, 3.0 h and 4.5 h after cells were treated with the reverse transcriptase inhibitor EFA at 12 h post infection. Here, TP53 is linked to infection.