There was no difference found in overall SAMHD1 levels (Fig. 4a and c), however both pSAMDH1 (T592) and RNR2 protein levels were elevated over multiple time points over HIV-1 infection in non-cycling HCT116 p53+/+ in comparison to non-cycling HCT116 p53−/− cells (Fig. 4a and d, e), even though the differences were not statistically significant. The gene discussed is SAMHD1; the disease is HIV-1 infection.