In the current study, the expression of Bcl-2, Bcl-xL, and Mcl-1 were found to be suppressed under the treatment of 7-AB in NCI-N87 cancer cell, while the levels of Atg5, Beclin 1, Bax, Bad, Bim were promoted, which demonstrated that 7-AB may induce apoptosis and autophagy through the dysfunction of mitochondria. This evidence concerns the gene MCL1 and cancer.