In brief, the experiment groups included: (1) I/R; (2) I/R + intraischemic cooling (I/R + IC); (3) I/R + delayed cooling R5 (DC-R5); (4) I/R + delayed cooling R10 (DC-R10); (5) I/R + delayed cooling R15 (DC-R15); (6) I/R + delayed cooling R20 (I/R + DC-R20); (7) I/R + DC-R20/baicalein (BC); (8) DC-R20/BC-C; (9) I/R + BC alone; (10) I/R + IC/BC; (11) DC-R20/BC + API-2 (an Akt inhibitor). This evidence concerns the gene AKT1 and breast cancer.