In the human BMBC, TGF-α was found to be expressed in the bone metastasis lesion, at a higher level than that in primary lesion of BMBC and Non-BM-BC (Figure 2), and TGF-α level in primary lesion of BMBC and Non-BM-BC was found to be higher than that in Benign control. The gene discussed is TGFA; the disease is breast cancer.