Another study performed immunohistochemistry analyses of several components of the Shh pathway on 80 surgical samples of NSCLC (stage I to III); a large majority of the samples were positive for Shh (97.5%), Ptch1 (78.7%), Smo (72.5%), Gli1 (98.7%) and Gli2 (87.5%) [5]. The gene discussed is GLI2; the disease is non-small cell lung carcinoma.