Thereby, to monitor and compare the autophagic flux during starvation time between siSCR cells and Gal-3-silenced cells (siGal-3), WM1366 melanoma cells transfected with mCherry-eGFP-LC3 were used to assess the formation rate of autophagosomes (AF, defined by both cherry- and GFP-puncta, i.e. yellow puncta) and autolysosomes (AL, defined by cherry only-puncta, since GFP is quenched in low pH). The gene discussed is LGALS3; the disease is melanoma.