Quantitative real-time PCR (qRT-PCR) showed that miR-125b expression was significantly lower in the NPM-ALK(+) ALCL cell lines KARPAS-299 and COST compared to non-tumorigenic CD4 lymphocytes both stimulated with CD3/CD28 antibodies (CD4.S, N = 3) or not (CD4.NS, N = 4) (Figure 1A). This evidence concerns the gene CD28 and anaplastic large cell lymphoma.