MYCN and neuroblastoma: Since, N-myc plays a primary role in the pathogenesis and aggressiveness of NB and PI3K/Akt pathway is known to stabilize N-myc protein, we first investigated the Akt/mTOR pathway proteins after treatment of both IMR 32 and GOTO cells with increasing concentrations (1–4 μM) of each withanolide (Figure 4).