To evaluate if the inhibitory effects of fasudil on AGEs-induced expression of EMT markers in cultured cells are reflected in vivo, TGF-β, FN, and α-SMA expression was assessed by immunohistochemistry and western blot in the peritoneum of rats with PD-related PF (Figure 7B). The gene discussed is TGFB1; the disease is pemphigus foliaceus.