The cut-off values for PicoGreen- andRiboGreen-adjusted IQGAP3 (14.5156 and 10.7485, respectively) that yielded thehighest combined sensitivity (80.0% and 84.4%, respectively) andspecificity (83.8% and 70.4%, respectively) for BC detection wereselected for further analysis. This evidence concerns the gene IQGAP3 and breast cancer.