Consistent with the ALI data demonstrating BC with high HMGA1 expression had a greater capacity to differentiate compared to BC with low HMGA1 expression (Figure 2), when HMGA1 was suppressed using the same siRNA strategy and the BC placed on ALI culture (Figure 3B), the expression of proliferation genes (MKI67, BIRC5), ciliated cell-related differentiation genes (FOXJ1, DNAI1) and secretory cell-related genes (SCGB1A1, MUC5B) were all suppressed compared with culture treated with control siRNA (Figure 3C). The gene discussed is DNAI1; the disease is breast cancer.