To test the effect of antibody treatment on signaling of Shh-mediated transcription factor GLI mRNA and protein expression levels, we performed qRT-PCR and Western blot analyses, respectively, with tumor lysates and observed a significant reduction in GLI transcripts (Figure 6A) and GLI polypeptide expression (Figure 6B) in samples, ex vivo, compared with the IgG controls. This evidence concerns the gene SHH and neoplasm.