SHH and plasma cell myeloma: Following dual intravenous (IV) administration of both KLH-conjugated peptides into Sp2/0-Ag14 mice to mount an immune response (Figure 1B), the B-cells from the isolated lymph nodes were fused to myeloma cells to generate a diverse repertoire of hybridomas (Figure 1C).We next performed protein-based screening analyses for anti-Shh C-term therapeutic antibody hybridoma candidates by capture ELISA, Western blotting and flow cytometry using both cancer cells and cells exogenously transfected for the expression of Shh (Figure 1D).