In ESCC tissue, we found that miR-1 was negatively correlated with Notch2 protein based on a Pearson correlation analysis (r = −0.713, p < 0.01) (Fig. 4D), but not with Notch2 mRNA (r = −0.202, p = 0.097) (Fig. 4E). This evidence concerns the gene NOTCH2 and esophageal squamous cell carcinoma.