To further confirm that PI4KIIα regulates insulin secretion, we overexpressed GFP-PI4KIIα and the kinase-dead mutant GFP-PI4KIIαK152A (Minogue et al.2006) in MIN6 cells (murine insulinoma-derived pancreatic β cell line), an insulin-secreting cell line (Ishihara et al.1993). This evidence concerns the gene INS and pancreatic insulinoma.