Since CTLA-4 is thought to be relevant in the context of T cell receptor engagement (Leach et al., 1996), we sought to determine the impact of the CD16a-V158F and CD32a-H131R SNPs, identified through sequencing of germline DNA, on response to ipilimumab in two separate cohorts of patients with advanced melanoma (Van Allen et al., 2015, Snyder et al., 2014) (Figure 5). This evidence concerns the gene CTLA4 and melanoma.