Herein, we evaluated the expression NKILA in laryngeal cancer tissues, assessed the functional role of NKILA in tumor cell proliferation, migration, apoptosis, and radioresistance to X‐ray radiation; moreover, we validated NKILA regulation of NF‐κB signaling and the combination of NKILA and NF‐κB: IκB complex, and further investigated whether NKILA affected laryngeal cancer radioresistance through NF‐κB signaling; finally, we investigated the mechanism by which NF‐κB regulated NKILA expression in tumor cells. This evidence concerns the gene NKILA and laryngeal carcinoma.