Colocalization between GPR50 and TβRI was observed in mouse brain slices of tanycytes, tanycyte primary cultures, human lung carcinoma NCI-H520 cells expressing endogenous GPR50, and in transfected HeLa cells as revealed by immunofluorescence microscopy and proximity ligation assay (PLA, Fig. 1b–d; Supplementary Fig. 1a-c). Here, TGFBR1 is linked to lung carcinoma.